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KMID : 0616120180490010055
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 1 p.55 ~ p.64
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Genetic Authentication of Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix by Using Conventional-PCR and Real-time PCR
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Ryu Hoe-Jin
Kim Ae-Kyung
Kim Sung-Dan
Chung Sam-Ju
Jang Jung-Im
Lee Hee-Jin
Lee Jeong-Mi
Yoo In-Sil
Jung Kweon
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Abstract
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Recently, it has been a big issue to distinguish the dried roots of Cynanchum wilfordii and C. auriculatum in health functional food market. The original plant species of Cynanchi Wilfordii Radix belong to the Asclepiadaceae family is differentially described in the national pharmacopoeia of Korea, China and Japan. Owing to the morphological similarities of the dried roots of this plant to those of C. auriculatum, which is often misidentified in Korean herbal medicine marketplace, distinguishing these two species is exceedingly difficult. The purpose of this study was to compare the conventional-PCR with the real-time PCR for detection of C. wilfordii and C. auriculatum DNA. We also tried to realize a quantitative real-time PCR assay using species-specific matK primers, which allowed us to estimate the ratio of C. willfordii and C. auriculatum using varying ratios of mixed genomic DNA template from the two species. The differentiation of intentional and unintentional mixture in this study would be applied to food safety management and can be helpful for protection of consumer’s right and cultivators.
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KEYWORD
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real-time PCR, conventional-PCR, CT(Cycle Threshold), Cynanchum wilfordii, Cynanchum auriculatum
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